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rabbit anti coup tf1  (Proteintech)


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    Proteintech rabbit anti coup tf1
    Rabbit Anti Coup Tf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+coup+tf1/NR2F1+Antibody/pmc03931022-469-165-164
    Average 93 stars, based on 16 article reviews
    rabbit anti coup tf1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Differential Loss of Prolyl Isomerase or Chaperone Activity of Ran-binding Protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis
    Article Snippet: .. Other antibodies used for immunofluorescence (IF) or immunoblots (IB) were as follows: rabbit anti-DsRed (1:1,500 (IB), Clontech); rat anti-HA (1:500 (IB), Roche Applied Science); mouse anti-HA (1:100 (IF), Abcam, Cambridge, MA); mouse anti-ubiquitin (1:1,000 (IB), Santa Cruz Biotechnology); rabbit anti-hsc70 (1:3,000 (IB), ENZO Life Science, Farmingdale, NY); monoclonal mouse anti-acetylated α-tubulin (1:40,000 (IB), Sigma); rabbit anti-L/M opsin no. 21069 (1:500 (IF), 1:1000 (IB)) ( 75 ); rabbit anti-S opsin (1:2,500 (IB), 1:100 (IF), JH455, gift from Jeremy Nathans); goat anti-S opsin (1:100 (IF) Millipore, Temecula, CA); peanut agglutinin TRITC conjugate (1:100 (IF), Sigma); rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:500 (IB), Santa Cruz Biotechnology); mouse mAb414 against nuclear pore complex proteins Nup62, Nup153, and Nup358 (400 ng/ml (IB), Covance, Emeryville, CA); mouse anti-Lamin A/C (1:1000 (IB), BD Biosciences); rabbit anti-19 S proteasome S1 (1:1000 (IB), Thermo Scientific, Rockford, IL); rabbit anti-hnRNPA2/B1 (1:1,500 (IB), 1:100 (IF), Proteintech, Chicago, IL); rabbit anti-HDAC4 (1:500 (IB), Santa Cruz Biotechnology); mouse anti-RANGAP1 (1:1,000 (IB), Invitrogen); rabbit anti-Nr2e3 (1:1,000 (IB), Proteintech); rabbit anti-COUP-TF1 (1:1,000 (IB), Abcam); rabbit anti-STAT3 (1:1000 (IB), Cell Signaling, Boston, MA); rabbit-STAT5 (1:1000 (IB), Abcam); rabbit phospho-STAT3 (1:1,000 (IB), Abcam); rabbit phospho-STAT5 (1:1000 (IB), Abcam); rabbit anti-γ-crystalline (gift from Vasanth Rao, originated by Dr. Sam Zigler); mouse PSR-45 (anti-phosphoserine antibody, 1:1000 (IB), Abcam ab6639); Alexa Fluor-conjugated secondary antibodies and Hoechst 33 342 were from Invitrogen. .. Co-immunoprecipitations and Western Analysis Nonidet P-40 extracts of transiently transfected HeLa cells were adjusted to 500 μg of protein per reaction and pre-cleared with nonimmune IgG and protein A/G-agarose (Santa Cruz Biotechnology) for 1 h at 4 °C.

    Article Title: Differential Loss of Prolyl Isomerase or Chaperone Activity of Ran-binding Protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis
    Article Snippet: .. Other antibodies used for immunofluorescence (IF) or immunoblots (IB) were as follows: rabbit anti-DsRed (1:1,500 (IB), Clontech); rat anti-HA (1:500 (IB), Roche Applied Science); mouse anti-HA (1:100 (IF), Abcam, Cambridge, MA); mouse anti-ubiquitin (1:1,000 (IB), Santa Cruz Biotechnology); rabbit anti-hsc70 (1:3,000 (IB), ENZO Life Science, Farmingdale, NY); monoclonal mouse anti-acetylated -tubulin (1:40,000 (IB), Sigma); rabbit anti-L/M opsin no. 21069 (1:500 (IF), 1:1000 (IB)) (75); rabbit anti-S opsin (1:2,500 (IB), 1:100 (IF), JH455, gift from Jeremy Nathans); goat anti-S opsin (1:100 (IF) Millipore, Temecula, CA); peanut agglutinin TRITC conjugate (1:100 (IF), Sigma); rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:500 (IB), Santa Cruz Biotechnology); mouse mAb414 against nuclear pore complex proteins Nup62, Nup153, and Nup358 (400 ng/ml (IB), Covance, Emeryville, CA); mouse anti-Lamin A/C (1:1000 (IB), BD Biosciences); rabbit anti-19 S proteasome S1 (1:1000 (IB), Thermo Scientific, Rockford, IL); rabbit anti-hnRNPA2/B1 (1:1,500 (IB), 1:100 (IF), Proteintech, Chicago, IL); rabbit anti-HDAC4 (1:500 (IB), Santa Cruz Biotechnology); mouse anti-RANGAP1 (1:1,000 (IB), Invitrogen); rabbit antiNr2e3 (1:1,000 (IB), Proteintech); rabbit anti-COUP-TF1 (1:1,000 (IB), Abcam); rabbit anti-STAT3 (1:1000 (IB), Cell Signaling, Boston, MA); rabbit-STAT5 (1:1000 (IB), Abcam); rabbit phospho-STAT3 (1:1,000 (IB), Abcam); rabbit phosphoSTAT5 (1:1000 (IB), Abcam); rabbit anti- -crystalline (gift from Vasanth Rao, originated by Dr. Sam Zigler); mouse PSR-45 (anti-phosphoserine antibody, 1:1000 (IB), Abcam ab6639); Alexa Fluor-conjugated secondary antibodies and Hoechst 33 342 were from Invitrogen. .. Co-immunoprecipitations and Western Analysis—Nonidet P-40 extracts of transiently transfected HeLa cells were adjusted to 500 g of protein per reaction and pre-cleared with nonimmune IgG and protein A/G-agarose (Santa Cruz Biotechnology) for 1 h at 4 °C.

    Western Blot:

    Article Title: Differential Loss of Prolyl Isomerase or Chaperone Activity of Ran-binding Protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis
    Article Snippet: .. Other antibodies used for immunofluorescence (IF) or immunoblots (IB) were as follows: rabbit anti-DsRed (1:1,500 (IB), Clontech); rat anti-HA (1:500 (IB), Roche Applied Science); mouse anti-HA (1:100 (IF), Abcam, Cambridge, MA); mouse anti-ubiquitin (1:1,000 (IB), Santa Cruz Biotechnology); rabbit anti-hsc70 (1:3,000 (IB), ENZO Life Science, Farmingdale, NY); monoclonal mouse anti-acetylated α-tubulin (1:40,000 (IB), Sigma); rabbit anti-L/M opsin no. 21069 (1:500 (IF), 1:1000 (IB)) ( 75 ); rabbit anti-S opsin (1:2,500 (IB), 1:100 (IF), JH455, gift from Jeremy Nathans); goat anti-S opsin (1:100 (IF) Millipore, Temecula, CA); peanut agglutinin TRITC conjugate (1:100 (IF), Sigma); rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:500 (IB), Santa Cruz Biotechnology); mouse mAb414 against nuclear pore complex proteins Nup62, Nup153, and Nup358 (400 ng/ml (IB), Covance, Emeryville, CA); mouse anti-Lamin A/C (1:1000 (IB), BD Biosciences); rabbit anti-19 S proteasome S1 (1:1000 (IB), Thermo Scientific, Rockford, IL); rabbit anti-hnRNPA2/B1 (1:1,500 (IB), 1:100 (IF), Proteintech, Chicago, IL); rabbit anti-HDAC4 (1:500 (IB), Santa Cruz Biotechnology); mouse anti-RANGAP1 (1:1,000 (IB), Invitrogen); rabbit anti-Nr2e3 (1:1,000 (IB), Proteintech); rabbit anti-COUP-TF1 (1:1,000 (IB), Abcam); rabbit anti-STAT3 (1:1000 (IB), Cell Signaling, Boston, MA); rabbit-STAT5 (1:1000 (IB), Abcam); rabbit phospho-STAT3 (1:1,000 (IB), Abcam); rabbit phospho-STAT5 (1:1000 (IB), Abcam); rabbit anti-γ-crystalline (gift from Vasanth Rao, originated by Dr. Sam Zigler); mouse PSR-45 (anti-phosphoserine antibody, 1:1000 (IB), Abcam ab6639); Alexa Fluor-conjugated secondary antibodies and Hoechst 33 342 were from Invitrogen. .. Co-immunoprecipitations and Western Analysis Nonidet P-40 extracts of transiently transfected HeLa cells were adjusted to 500 μg of protein per reaction and pre-cleared with nonimmune IgG and protein A/G-agarose (Santa Cruz Biotechnology) for 1 h at 4 °C.

    Article Title: Differential Loss of Prolyl Isomerase or Chaperone Activity of Ran-binding Protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis
    Article Snippet: .. Other antibodies used for immunofluorescence (IF) or immunoblots (IB) were as follows: rabbit anti-DsRed (1:1,500 (IB), Clontech); rat anti-HA (1:500 (IB), Roche Applied Science); mouse anti-HA (1:100 (IF), Abcam, Cambridge, MA); mouse anti-ubiquitin (1:1,000 (IB), Santa Cruz Biotechnology); rabbit anti-hsc70 (1:3,000 (IB), ENZO Life Science, Farmingdale, NY); monoclonal mouse anti-acetylated -tubulin (1:40,000 (IB), Sigma); rabbit anti-L/M opsin no. 21069 (1:500 (IF), 1:1000 (IB)) (75); rabbit anti-S opsin (1:2,500 (IB), 1:100 (IF), JH455, gift from Jeremy Nathans); goat anti-S opsin (1:100 (IF) Millipore, Temecula, CA); peanut agglutinin TRITC conjugate (1:100 (IF), Sigma); rabbit anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody (1:500 (IB), Santa Cruz Biotechnology); mouse mAb414 against nuclear pore complex proteins Nup62, Nup153, and Nup358 (400 ng/ml (IB), Covance, Emeryville, CA); mouse anti-Lamin A/C (1:1000 (IB), BD Biosciences); rabbit anti-19 S proteasome S1 (1:1000 (IB), Thermo Scientific, Rockford, IL); rabbit anti-hnRNPA2/B1 (1:1,500 (IB), 1:100 (IF), Proteintech, Chicago, IL); rabbit anti-HDAC4 (1:500 (IB), Santa Cruz Biotechnology); mouse anti-RANGAP1 (1:1,000 (IB), Invitrogen); rabbit antiNr2e3 (1:1,000 (IB), Proteintech); rabbit anti-COUP-TF1 (1:1,000 (IB), Abcam); rabbit anti-STAT3 (1:1000 (IB), Cell Signaling, Boston, MA); rabbit-STAT5 (1:1000 (IB), Abcam); rabbit phospho-STAT3 (1:1,000 (IB), Abcam); rabbit phosphoSTAT5 (1:1000 (IB), Abcam); rabbit anti- -crystalline (gift from Vasanth Rao, originated by Dr. Sam Zigler); mouse PSR-45 (anti-phosphoserine antibody, 1:1000 (IB), Abcam ab6639); Alexa Fluor-conjugated secondary antibodies and Hoechst 33 342 were from Invitrogen. .. Co-immunoprecipitations and Western Analysis—Nonidet P-40 extracts of transiently transfected HeLa cells were adjusted to 500 g of protein per reaction and pre-cleared with nonimmune IgG and protein A/G-agarose (Santa Cruz Biotechnology) for 1 h at 4 °C.



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    Figure 1. Immunohistochemical analysis of Shh pathway expression in human brain AVMs. Representative images of human brain AVMs and control brain vessels. Cells in the intimal layer of AVMs are strongly immunopositive for Shh and Gli1. <t>COUP-TFII</t> immunopositive cells are detectable in the intimal and medial layers of AVMs. Control brain vessels are negative in terms of Shh, Gli1, and COUP-TFII expression. Shh: Sonic hedgehog.
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    rabbit anti coup tf1  (Proteintech)
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    Proteintech rabbit anti coup tf1
    Figure 1. Immunohistochemical analysis of Shh pathway expression in human brain AVMs. Representative images of human brain AVMs and control brain vessels. Cells in the intimal layer of AVMs are strongly immunopositive for Shh and Gli1. <t>COUP-TFII</t> immunopositive cells are detectable in the intimal and medial layers of AVMs. Control brain vessels are negative in terms of Shh, Gli1, and COUP-TFII expression. Shh: Sonic hedgehog.
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    Image Search Results


    Figure 1. Immunohistochemical analysis of Shh pathway expression in human brain AVMs. Representative images of human brain AVMs and control brain vessels. Cells in the intimal layer of AVMs are strongly immunopositive for Shh and Gli1. COUP-TFII immunopositive cells are detectable in the intimal and medial layers of AVMs. Control brain vessels are negative in terms of Shh, Gli1, and COUP-TFII expression. Shh: Sonic hedgehog.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Sonic hedgehog is expressed in human brain arteriovenous malformations and induces arteriovenous malformations in vivo

    doi: 10.1177/0271678x20912405

    Figure Lengend Snippet: Figure 1. Immunohistochemical analysis of Shh pathway expression in human brain AVMs. Representative images of human brain AVMs and control brain vessels. Cells in the intimal layer of AVMs are strongly immunopositive for Shh and Gli1. COUP-TFII immunopositive cells are detectable in the intimal and medial layers of AVMs. Control brain vessels are negative in terms of Shh, Gli1, and COUP-TFII expression. Shh: Sonic hedgehog.

    Article Snippet: For the detection of COUP-TFII expression in brain vasculature, sections were incubated with lectin from Lycopersicon esculentum (tomato) biotin conjugate (1:500) (SigmaAldrich, St. Louis, MO, USA) together with a rabbit polyclonal anti-COUP-TFII antibody (Origene, Rockville, MD, USA).

    Techniques: Immunohistochemical staining, Expressing, Control

    Figure 7. Expression of COUP-TFII in phShh-induced brain neovessels. Sections of brain hemispheres injected with phShh and empty plasmid were stained for DAPI (blue staining) to identify cell nuclei, lectin (red staining) to identify blood vessels, and COUP- TFII (green staining) to identify cells expressing this vascular differentiation marker. (a) COUP-TFII-positive cells were found in the neovessels grown in the brain in response to phShh injection, both at the level of the intimal layer and the vascular wall. (b) COUP- TFII-positive cells were virtually absent in brain hemispheres injected with the empty plasmid.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Sonic hedgehog is expressed in human brain arteriovenous malformations and induces arteriovenous malformations in vivo

    doi: 10.1177/0271678x20912405

    Figure Lengend Snippet: Figure 7. Expression of COUP-TFII in phShh-induced brain neovessels. Sections of brain hemispheres injected with phShh and empty plasmid were stained for DAPI (blue staining) to identify cell nuclei, lectin (red staining) to identify blood vessels, and COUP- TFII (green staining) to identify cells expressing this vascular differentiation marker. (a) COUP-TFII-positive cells were found in the neovessels grown in the brain in response to phShh injection, both at the level of the intimal layer and the vascular wall. (b) COUP- TFII-positive cells were virtually absent in brain hemispheres injected with the empty plasmid.

    Article Snippet: For the detection of COUP-TFII expression in brain vasculature, sections were incubated with lectin from Lycopersicon esculentum (tomato) biotin conjugate (1:500) (SigmaAldrich, St. Louis, MO, USA) together with a rabbit polyclonal anti-COUP-TFII antibody (Origene, Rockville, MD, USA).

    Techniques: Expressing, Injection, Plasmid Preparation, Staining, Marker